Combined Peroxidase Reaction and Paragon Staining of Epoxy-Embedded Tissue Sections

Application of standard micro-anatomical staining methods to epoxy resin-embedded sections.

Staining of semi-thin sections of osmium-fixed epoxy resin (Epon or Araldite)-embedded tissues with haematoxylin and acid dyes as counterstains (eosin or phloxin) was successfully achieved after intense oxidation using hydrogen peroxide as the oxygen donor. Results are closely similar to those obtained with paraffin sections (haematoxylin and eosin): nuclei stain blue, cytoplasm and extracellul...

Oxidized p-phenylenediamine staining of epoxy resin sections.

Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable ele...

Non-nuclear staining of thick tissue sections.

A Staining Method for Sections of Osmium-Fixed, Methacrylate-Embedded Tissue

The increasingly broad application of electron microscopy to the study of cytological detail in both normal and pathological tissues has created a need for an improved staining method for light microscopy of material processed primarily for electron optical investigation. A staining technic that would produce results comparable to those obtained with hematoxylin and eosin should prove to be of ...

Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material.

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...